Te whakamahinga o te hangarau whakamatao hiko wera i roto i ngā taputapu PCR

Te whakamahinga o te hangarau whakamatao hiko wera i roto i ngā taputapu PCR

Ko te whakamahinga o te hangarau whakamatao hiko wera i roto i ngā taputapu PCR kei roto i te whakahaere pāmahana. Ko tōna painga matua ko te kaha whakahaere pāmahana tere me te tika, e whakarite ana i te reiti angitu o ngā whakamātautau whakanui DNA.

Ngā horopaki tono matua

1. Te whakahaere pāmahana tika

Me huri te taputapu PCR mā roto i ngā wāhanga e toru: te whakakorenga pāmahana teitei (90-95℃), te whakamahana pāmahana iti (55-65℃), me te toronga pāmahana tino pai (70-75℃). He uaua ki te whakatutuki i te whakaritenga tika o te ±0.1℃ ngā tikanga whakamatao tuku iho. Mā te whakamatao thermoelectric, ka tutuki i te hangarau whakamatao peltier te whakahaere pāmahana taumata mirihekona mā te pānga Peltier, ka karohia te rahunga whakanui i puta mai i te rerekētanga pāmahana 2℃.

2. Te whakamatao me te whakamahana tere

Ka taea e ngā kōwae whakamatao hiko werawera, ngā kōwae hiko werawera, ngā taputapu peltier, ngā kōwae peltier te whakatutuki i te tere whakamatao o te 3 ki te 5 nekehanga Celsius ia hekona, ka whakapotohia te huringa whakamātautau ki te 2 nekehanga Celsius ia hekona o ngā pūwhakakoi tuku iho. Hei tauira, ka whakamahia e te taputapu PCR 96-puna te hangarau whakahaere pāmahana ā-rohe hei whakarite i te pāmahana ōrite i roto i ngā tūranga puna katoa, me te karo i te rerekētanga pāmahana 2℃ i puta mai i ngā pānga taha.

3. Whakapai ake i te pono o ngā taputapu

Ko ngā kōwae whakamatao hiko wera, ngā kōwae peltier, ngā wāhanga peltier, ngā kōwae TEC o Beijing Huimao Cooling Equipment Co., Ltd. kua noho hei wāhanga matua o te whakahaere pāmahana o ngā taputapu PCR nā te mea he tino pono. Nā te iti o te rahi me ngā āhuatanga kore haruru, he pai mō ngā whakaritenga tika o ngā taputapu hauora.

Ngā take tono noa

Pūoko PCR tauanga fluorescence 96-puna: Kua whakauruhia ki te kōwae whakamatao thermoelectric, te kōwae TEC, te taputapu peltier, ngā kōwae peltier, ka taea te whakahaere tika i te pāmahana o ngā tauira whakaputanga-teitei, ā, e whakamahia whānuitia ana i roto i ngā mara pēnei i te tātari whakaaturanga ira me te kimi mate.

Ngā pouaka whakamātao hauora kawe: ngā pouaka whakamātao hiko wera, ngā pouaka whakamātao peltier e whakamahia ana hei penapena i ngā hua pēnei i ngā kano kano me ngā rongoā e hiahia ana ki te taiao pāmahana iti, kia mau ai te pumau o te pāmahana i te wā e kawe ana.

Ngā taputapu maimoatanga taiaho:

Ka whakamataohia te kaituku taiaho e ngā kōwae whakamatao hiko werawera, ngā huānga peltier, ngā kōwae hiko werawera hei whakaiti i te mōrearea o te wera kiri, hei whakarei ake hoki i te haumaru maimoatanga.

Whakatakotoranga TEC1-39109T200

Ko te pāmahana taha wera he 30 C,

Atamira: 9A

Umax: 46V

Qmax：246.3W

ACR: 4±0.1Ω（Ta= 23 C）

Teitei T teitei: 67 -69C

Rahi: 55x55x3.5-3.6mm

Ngā Whakatakotoranga TES1-15809T200

Te pāmahana taha wera: 30 C,

Imax: 9.2A

Umamax: 18.6V

Qmax：99.5 W

Teitei T teitei: 67 C

ACR：1.7 ±15% Ω （1.53 ki te 1.87 Ohm）

Rahi: 77×16.8×2.8mm

Waea: Waea silicone 18 AWG, he rite rānei ki te Sn-whakakikoruatia ki te mata, ātete pāmahana teitei 200℃